Production of extracellular polysaccharides and phycobiliproteins from Tolypothrix sp. PCC7601 using mechanical milking systems

Algal Research, in press 2020

Alice Uchida, Yukiko Higashi, Shota Yamamoto, Jun Nakanishi, Naoki Kanayama, Kazuhiro Shibata, Masaki Ihara

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A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells
2019
Author:Mitsuyoshi Miyata, Shunsuke Iwata, Chie Mifude, Kazuyuki Matsushima, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda

Overexpression of endogenous 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation
Journal of Bioscience and Bioengineering,123(5):590-596 2017
Author:Kai Kudoh, Shingo Hotta, Midori Sekine, Arisu Uchida, Genma Kubota, Rintaro Fujii, Yusuke Kawano, Masaki Ihara

Exploration of the 1-deoxy-D-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system
Journal of Bioscience and Bioengineering,123(3):300-307 2017
Author:Kudoh Kai, Kubota Genma, Fujii Rintaro, Kawano Yusuke, Ihara Masaki*

Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
Applied Microbiology and Biotechnology,101(1):341-349 2017
Author:Shigemori S, Ihara M, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T

 

生物触媒を利用した半人工光合成
化学と工業,69(11):963-965 2016
Author:伊原正喜

Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice
Microbial Cell Factories,14:189 2015
Author:Suguru Shigemori, Takafumi Watanabe, Kai Kudoh, Masaki Ihara, Shireen Nigar, Yoshinari Yamamoto, Yoshihito Suda, Takashi Sato, Haruki Kitazawa* and Takeshi Shimosato*

Expression Systems for Soluble Metal-Dependent Formate Dehydrogenase
Journal of Photochemistry & Photobiology, A: Chemistry 2015

Enhancement of L-cysteine production by disruption of yciW in Escherichia coli
Journal of Bioscience and Bioengineering,119:176-179 2015

Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases
Mol. Biotechnol,56:953-961 2015

微細藻類によるバイオエネルギー及びバイオマテリアル生産
月刊技術士,595(9):12-15 2014
Author:伊原正喜

Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-D-xylulose 5-phosphate synthase and carbon allocation analysis
Journal of Bioscience and Bioengineering,118:20-28 2014

Identification and characterization of UDP-glucose pyrophosphorylase in Anabaena sp. PCC 7120
Journal of Bioscience and Bioengineering,117:531-538 2014

Expression of an antibody-enzyme complex by the L-chain fusion method
Journal of Bioscience and Bioengineering,116:17-21 2013
Author:Chungyong Han, Masaki Ihara, and Hiroshi

A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases
Analytical Chemistry,85:7985-7940 2013

Light Driven CO2 Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
PLOS ONE,8(8):e71581 2013
Author:Ihara, M; Kawano, Y; Urano, M; Okabe, A

Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments
Bioconjugate Chemistry,23:1463-1469 2012
Author:Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, and Hiroshi Ueda

“Quenchbodies”: quench-based antibody probes that show antigen-dependent fluorescence
J. Am. Chem. Soc.,133:17386 2011
Author:R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka and H. Ueda

Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules
Bioconjugate chemistry,:633-641 2011
Author:Kojima et al.

Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4
Analytical Chemistry,83:1008-1014 2011
Author:Islam et al.

Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells
Journal of Immunological Methods,361:57-63 2010
Author:SasaJima et al.

Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
Bioanalysis,2:1683-1687 2010
Author:Islam et al.

Antibody affinity maturation in vitro using unconjugated peptide antigen
Protein Engineering Design and Selection,23:185-193 2010
Author:Iwai et al.

Antibody affinity maturation in vitro using unconjugated peptide antigen
Lab on a Chip,10:92-100 2010
Author:Ihara et al.

Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol
Analytical Chemistry,81:8298-8304 2009
Author:Ihara et al.

Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen
Analytical Chemistry,81:7532-7537 2009
Author:Sakata et al.

Trans-splicing as a novel method to rapidly produce antibody fusion proteins
Biochemical and Biophysical Research Communications,384:316-321 2009
Author:Iwasaki et al.

Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology, Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils
Biophysical Journal,96:3331-3340 2009
Author:Zako et al.

Antibody Fab display system that can perform open sandwich ELISA
Analytical Biochemistry,386:36-44 2009
Author:Dong et al.

Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase
Photochemical andPhotobiology,82:1677-1685 2006
Author:Ihara et al.

Design of Artificial Hydrogenase-Photosystem I Complex for Photo-driven Hydrogen Production by Cyanobacteria or Microalga
Photochemical and Photobiology,82:676-682 2006
Author:Ihara et al.

Functions of fluctuation in the heme-binding loops of cytochrome b5 revealed in the process of heme incorporation
2000
Author:Masaki Ihara, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima

Conversion of an electron-transfer protein into an oxygen binding protein: The axial cytochrome b5 mutant with an unusually high O2 affinity
2000
Author:Masaki Ihara, Masato Shintaku, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima